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  • 5× SDS-PAGE Loading Buffer
  • 產品型號:20078

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For Protein SDS-PAGE Electrophoresis

For Protein SDS-PAGE Electrophoresis

Components:
250mM    Tris-HCl(pH6.8)
10%(W/V)    SDS
0.5%(W/V)    BPB
50%(V/V)    Glycerol

5X SDS-PAGE Loading Buffer is ideal for preparing protein samples to be separated in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and allows for easy sample monitoring during electrophoresis.

SDS-PAGE Loading Buffer also protects proteins from heat degradation during the sample preparation step, as well against pH changes during the SDS-PAGE run. Some proteins are sensitive to pH changes that result from temperature fluctuations during electrophoresis in Tris buffers. The optimized composition of the loading buffer prevents protein degradation during sample heating prior to SDS-PAGE as well as during the electrophoresis run.

Gently mix 50ul 2-ME and 1mL SDS-PAGE Loading Buffer to make the working 5x Loading Buffer before using.

Store at 4℃, or -20℃ for a long period. The Loading buffer added 2-ME could be stable at RT for about one month.