生工gCut Cas9
精準剪切熊厲害


Streptococcus pyogenes Cas9
內毒素小於10 EU/mg,可直接用於注射實驗。
C端融合NLS,精準導向細胞核。


gCut Cas9-NLS Nuclease is the recombinant Streptococcus pyogenes Cas9 protein, purified from E. coli, and edit genome by inducing site-specific double stranded breaks.

gCut Cas9-NLS Nuclease forms a very stable ribonucleoprotein (RNP) complex with the short guide RNA (sgRNA). Incorporation of nuclear localization signals (NLS) make Cas9 nuclease able to deliver to the nucleus then bind to sgRNA directly. The Cas9 RNP complex is rapidly cleared from the cell minimizing the chance of off-target cleavage when compared to other systems. This DNA-free system avoids the risk of inserting foreign DNA into the genome.

gCut Cas9-NLS Nuclease contains a NLS on the C-terminus of the protein to meet all the researchers’ requirements (e.g. in vitro cleavage assay, RNP complex transfection, and micro injection). With gCut Cas9-NLS Nuclease, customers can edit gene efficiently by this powerful tool.

Features:
Applications:
Components:
Component Quantity Storage
gCut Cas9-NLS Nuclease 50 μg -20℃ for 2 years
10X Reaction Buffer 1.5 mL -20℃
DEPC Treated Nuclease-Free Water 1.0 mL -20℃, 4℃ or RT
Specifications:
Product Code 5700100050
Expression E. coli
Gene Aliases csn1
Gene ID 901176
Product Size 50 μg, 3.2 mg/ml (20 μM)
Protein Form Recombinant
Protein Tag 6X His
Shipping Condition Dry ice
Quality Control:


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