檔案名稱：30000002.htm
GAPDH, Rabbit Polyclonal Antibody
100uL(100ug), Lyophilized
高效價，低售價
每管100uL(100ug)，僅售2310元。
| Product Code: | 30000002 |
| Background: | Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)
is one of the key enzymes involved in glycolysis. It catalyzes the
reversible oxidative phosphorylation of glyceraldehydes-3-phosphate. GAPDH
is composed of four 30-40kDa subunits. The molecular weight for GAPDH is
146kDa. The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a “housekeeping” gene. Housekeeping proteins like GAPDH are used as loading controls for western blots and protein normalization. |
| Production: | Produced by immunizing rabbits with a recombinant protein mapping at the carbonic terminus of GAPDH of human origin and isolated from antisera by immunoaffinity chromatography. |
| Specificity: | Recommended for the detection of GAPDH of mouse, rat and human origin. |
| Reconstitution: | Reconstitute lyophilized powder in 100uL ddH2O. |
| Storage: | Store lyophilized powder at 4 or -20℃. After reconstitution, store at -20℃ or below and avoid freeze-thawing cycles. |
| Shipping: | Room Temp. |
| Application | Dilution Factor |
| Immunohistochemistry | 100-200 |
| Immunofluorescence | 100-200 |
| Western Blot | 1,000-2,000 |
| ELISA | 15,000-30,000 |
| Optimal conditions must be determined individually for each application. | |
| QC Data: | Western blot analysis of GAPDH expression
in 293A (A) and Hela (B) whole cell lysates and mouse kidney tissue
extract (C) |
採購找生工，預算最輕鬆。
中華民國98年11月20日更新