LOCUS       Exported                1823 bp ds-DNA     circular SYN 24-MAR-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pUC57Amp1.8k
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   MDBio, Inc.
  TITLE     Direct Submission
  JOURNAL   Exported Friday, March 24, 2017 from SnapGene Viewer 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          102..101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(169..757)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(928..1788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1789..70)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
       61 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgatatcaca tgtgagcaaa
      121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1801 tcaatattat tgaagcattt atc
//